CRM197
Catalog Number
CRM-197
Description
CRM197 diphtheria toxin mutant, recombinant. Pre-clinical grade.
Product Background
CRM197 is a non-toxic mutant of diphtheria toxin1,2 having a single amino acid substitution of glutamic acid for glycine. CRM197 is a well defined protein and functions as a carrier for polysaccharides and haptens making them immunogenic. It is utilized as a carrier protein in a number of approved conjugate vaccines for diseases such as meningitis and pneumococcal bacterial infections. Pfenex CRM197 is a recombinant form expressed in Pseudomonas fluorescens using the Pfēnex Expression TechnologyTM platform.
This product is available in both pre-clinical and cGMP grade.
This product is available in both pre-clinical and cGMP grade.
Category
Vaccine Components
Source
Pseudomonas fluorescens
Species
Corynebacterium diphtheriae
Formulation
CRM197 is lyophilized from a 0.2 um-filtered solution containing 4 mg/mL CRM197, 50 mg/mL sucrose, 0.22 mg/mL sodium phosphate monobasic, 3 mg/mL sodium phosphate dibasic, 0.055 mg/mL polysorbate 80.
Product Molecular Mass: 58.4 kDa
Product Molecular Mass: 58.4 kDa
Storage
Store at 2-8 °C upon receipt
Purity
Purity: > 95% CRM197 by SDS-PAGE
A and B chain content < 5% of total CRM197
Endotoxin: < 100 EU/mg of protein by LAL method
Dimer: < 5%
A and B chain content < 5% of total CRM197
Endotoxin: < 100 EU/mg of protein by LAL method
Dimer: < 5%
Uses
Before use, aseptically reconstitute lyophilized CRM197 with sterile deionized water to a concentration of 4 mg/mL CRM197. Reconstituted CRM197 should be stored at 2-8 °C.
References
1. Giannini, G., Rappuoli, R., and Ratti, G. Nucleic Acids Res. 12: 4063-4069 (1984)
2. Mekada, E., and Uchida, T. J. Biol. Chem. 260: 12148-12153 (1985)
2. Mekada, E., and Uchida, T. J. Biol. Chem. 260: 12148-12153 (1985)
