Product Details

CRM197

Catalog Number

CRM-197

Description

CRM197 diphtheria toxin mutant, recombinant. Pre-clinical grade.

Product Background

CRM197 is a non-toxic mutant of diphtheria toxin1,2 having a single amino acid substitution of glutamic acid for glycine. CRM197 is a well defined protein and functions as a carrier for polysaccharides and haptens making them immunogenic. It is utilized as a carrier protein in a number of approved conjugate vaccines for diseases such as meningitis and pneumococcal bacterial infections. Pfenex CRM197 is a recombinant form expressed in Pseudomonas fluorescens using the Pfēnex Expression TechnologyTM platform.

This product is available in both pre-clinical and cGMP grade.

For more information on CRM197 or our other vaccine carriers, please contact us or visit our knowledge center for articles, publications and other resources on CRM197 and our other vaccine carriers.


Category

Vaccine Components

Source

Pseudomonas fluorescens

Species

Corynebacterium diphtheriae
Formulation
CRM197 is lyophilized from a 0.2 um-filtered solution containing 4 mg/mL CRM197, 50 mg/mL sucrose, 0.22 mg/mL sodium phosphate monobasic, 3 mg/mL sodium phosphate dibasic, 0.055 mg/mL polysorbate 80.

Product Molecular Mass: 58.4 kDa

Storage

Store at 2-8 °C upon receipt

Purity

Purity: > 95% CRM197 by SDS-PAGE
             A and B chain content < 5% of total CRM197

Endotoxin: < 100 EU/mg of protein by LAL method

Dimer: < 5%

Uses

Before use, aseptically reconstitute lyophilized CRM197 with sterile deionized water to a concentration of 4 mg/mL CRM197. Reconstituted CRM197 should be stored at 2-8 °C.

References

1. Giannini, G., Rappuoli, R., and Ratti, G. Nucleic Acids Res. 12: 4063-4069 (1984)
2. Mekada, E., and Uchida, T. J. Biol. Chem. 260: 12148-12153 (1985)
SIZE
PRICE
1.00 mg
 
$475.00
10.00 mg
 
$4,000.00
20.00 mg
 
$6,500.00
Quantity
Data Sheet
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Amino Acid Sequence
GADDVVDSSK SFVMENFSSY HGTKPGYVDS IQKGIQKPKS GTQGNYDDDW KEFYSTDNKY DAAGYSVDNE NPLSGKAGGV VKVTYPGLTK VLALKVDNAE TIKKELGLSL TEPLMEQVGT EEFIKRFGDG ASRVVLSLPF AEGSSSVEYI NNWEQAKALS VELEINFETR GKRGQDAMYE YMAQACAGNR VRRSVGSSLS CINLDWDVIR DKTKTKIESL KEHGPIKNKM SESPNKTVSE EKAKQYLEEF HQTALEHPEL SELKTVTGTN PVFAGANYAA WAVNVAQVID SETADNLEKT TAALSILPGI GSVMGIADGA VHHNTEEIVA QSIALSSLMV AQAIPLVGEL VDIGFAAYNF VESIINLFQV VHNSYNRPAY SPGHKTQPFL HDGYAVSWNT VEDSIIRTGF QGESGHDIKI TAENTPLPIA GVLLPTIPGK LDVNKSKTHI SVNGRKIRMR CRAIDGDVTF CRPKSPVYVG NGVHANLHVA FHRSSSEKIH SNEISSDSIG VLGYQKTVDH TKVNSKLSLF FEIKS
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